目的 利用分子印迹技术制备槲皮素分子印迹聚合物（MIPs）。方法 以槲皮素为模板分子，丙烯酰胺为功能单体，乙二醇二甲基丙烯酸酯为交联剂，偶氮二异丁腈为引发剂，分别在无水乙醇和四氢呋喃为致孔剂的作用下，采用沉淀聚合法、本体聚合法制备槲皮素MIPs；利用紫外光谱、红外光谱选择模板分子与功能单体间的最佳配比；通过扫描电镜（SEM）考察了MIPs的微观结构。采用平衡和等温吸附实验对2种方法制备的MIPs的吸附平衡时间和最大吸附量进行考察，并对特异性吸附能力进行研究。结果 沉淀聚合法制备的槲皮素MIPs具有均匀规则的球状结构，在吸附动力学和等温吸附实验中发现有较快的吸附速度和较大的吸附量，在芦丁和槲皮素的选择性吸附过程中聚合物对槲皮素具有较高的特异性识别能力。结论 沉淀聚合法制备的MIPs以吸附量大、选择性强，为中药黄酮类复杂化学成分的分离、富集提供一种新的研究方法，同时也为其他中药化学成分的研究提供借鉴。

Objective To prepare quercetin molecularly imprinted polymers (MIPs) by molecular imprinting technique. Methods This experiment used quercetin as template molecule, acrylamide (AM) as functional monomers, ethylene glycol dimethacrylate (EGDMA) as cross linking agent, and azodiisobutyronitrile (AIBN) as initiator. Using anhydrous ethanol and tetrahydrofuran as pore forming agent, quercetin MIPs were prepared by precipitation polymerization and mass polymerization methods; The optimal proportion between template and functional monomer was chosen by UV and IR; The microstructure of MIPs was investigated by SEM. The adsorption equilibrium time and maximum adsorption of MIPs prepared with two methods were investigated by balance and isothermal adsorption experiment, and the specific adsorption capacity was studied. Results The experimental research showed that precipitation polymerization method of quercetin MIPs had uniform rules of globular structures. The adsorption kinetics and isothermal adsorption experiment found faster adsorption rate and larger adsorption capacity. Polymer on quercetin had high specificity recognition ability in rutin and quercetin adsorption selective process. Conclusion Precipitation polymerization of MIPs, with strong selective adsorption, separation and enrichment of chemical components in Chinese medicine flavonoids complex, is a new method of research. At the same time, it also provides reference for chemical composition research of other Chinese materia medica.

国家自然科学基金资助项目（81460595）；江西中医药大学校级研究生创新专项资金项目（JZYC16S35）