目的 分析蕲蛇药材正品及其市场收集样品的Cyt b基因序列,为蕲蛇药材分子鉴别提供依据。方法 利用Cyt b基因,对蕲蛇药材及其市场收集样品进行了序列测定和分析。结果 蕲蛇药材存在着混杂现象;蕲蛇药材正品与其市售混淆品的Cyt b基因序列有着显著差异,该序列在蕲蛇种内差异率为0%~0.91%,与混淆品间的差异率为18.57%~23.78%。结论 Cyt b基因序列是一种鉴别蕲蛇药材与其混淆品较好的分子遗传标记。

Objective To differentiate the sequences of Cyt b gene Agkistrodon from its adulterants sold in the market in order to provide molecular evidence for identification of Agkistrodon. Methods Cyt b gene was used to sequence and analyze Agkistrodon and its adulterants sold in the market. Results There was a mixed phenomenon in Agkistrodon species. The differences of Cyt b gene sequence between Agkistrndnn and its adulterants sold in the market are significant:the difference. rates among the species of Agkistrodon are 0%-0.91%，the difference rates between Agkistrodon and its adulterants are 18. 57%一23. 78 %. Conclusion The characteristics of Cyt b gene sequence can be used as a better molecular marker for authenticating Agkistrodon from its adulterants.

安徽省自然科学基金资助项目(03043101);安徽省优秀青年科技基金资助项目(04043056)