| [Abstract] Objective The biodegradation, bioactivity, compressive strength, biocompatibility in vitro and the bone repairing ability in vivo of D-Alk-2B bioactive glass scaffolds were investigated, which were prepared in our lab and have a microstructure similar to that of human trabecular bone. Methods ① D-Alk-2B scaffolds were soaked in 0.02 M K2HPO4 solution to measure the changes in weight loss, compressive strength and pH value of soaking solution with the time. ② D-Alk-2B scaffolds seeded with MLO-A5 cells were cultured with different time to measure the cell viability, alkaline phosphatase activity assay and cell adhesion of the scaffolds. ③ D-Alk-2B scaffolds was implanted into subcutaneous pockets in the dorsum of the rats to evaluate the bone repairing ability. Results During immersed in K2HPO4 solution, the D-Alk-2B scaffolds gradually degraded and converted to Hydroapatite (HA), and the compressive strength of them decreased, while the pH value of solution increased with soaking time. D-Alk-2B scaffolds could support the proliferation and differentiation of MLO-A5 cells, have good ALP activity and MLO-A5 cells could attach on the surface of the scaffolds. After implanted in rat subcutaneous, the scaffold could support soft tissue infiltration. Conclusion D-Alk-2B bioactive glass scaffolds prepared in our lab have excellent biodegradation, bioactivity, biocompatibility, and have osteoconductivity and osteoinductivity, which would be a novel bone tissue engineering material having wildly prospect and clinic applications. |
