Objective: To synthesize galactose ligand in non-aqueous medium by enzyme-catalyzed method. Method: Structure of the product was confirmed by IR,ESI-MS,¹H-NMR and¹C-NMR;With conversion rate of substrate as index,effects of enzyme species,reaction medium,the amount of enzyme,molar ratio of substrate,reaction temperature on ligand synthesis was investigated by single factor tests. Result: Characterization by catalytic reaction,C1' bit hydroxyl of galactose occurred esterification,the received products was the target product.Optimized reaction conditions were as followings:tetrahydrofuran(THF)-dimethyl sulfoxide(DMSO)3:1 as reaction medium,Novozym 435 immobilized lipase as catalyst,the amount of enzyme of 600 U·mL^-1,molar ratio of galactose to vinyl stearate at 1:4,and reacted at 55 ℃ for 8 h,the conversion rate of galactose reached more than 70%. Conclusion: Enzyme catalysis method could be used for synthesizing ligand of liver targeted liposomes.