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[摘要]
目的 探讨乙烯利、乙酰水杨酸、色氨酸3种试剂对长春花植株中药用成分积累的影响。方法 用不同质量浓度的3种试剂溶液分别处理温室盆栽长春花植株,2d后,测定叶片中的总吲哚生物碱含量,并采用RP-HPLC法测定长春碱和长春质碱的含量。结果 3种试剂处理后吲哚总碱、长春碱、长春质碱的积累均有明显的提高,并确定了最适的处理质量浓度。结论 3种试剂对长春花植物中吲哚生物碱的产生有明显的促进作用。
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[Abstract]
Objective To study the effect of three kinds of reagent:ethrel,Aspirin,tryptophan on the accumulation of medicinal component in Catharanthus roseus leaves.Methods To treat these potplants of C.roseus in green house with the three reagents in different concentrations,respectively.Then,two days later,the contents of catharanthine and vinblastine in leaves were determined by RP-HPLC.Results The three reagents can obviously improve the accumulation of indole alkaloids,including catha-ranthine and vinblastine in C.roseus leaves.Their optimum treatment concentration was finally corn-firmed.Conclusion These three kinds of reagent apparently promote the accumulation of total indole alkoloids in the leaves of C.roseus
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