Aim To evaluate whether the value of 64-detector spiral computed tomography coronary angiography （64-DSCTCA） is identical between female and male in diagnosis of patients with coronary artery stenosis. Methods 75 female and 75 male patients with coronary artery disease （CAD） or suspected CAD were chosen, who had been examined with not only 64-DSCTCA but also coronary angiography （CAG）. The result of CAG was regarded as the gold standard. The sensitivities, specificities, positive prediction values and negative prediction values of 64-DSCTCA were respectively calculated in diagnosis of female and male patients with CAD （coronary artery stenosis≥50%）, coronary artery moderate stenosis （stenosis 50%～75%）, severe stenosis and occlusion （stenosis 76%～100%）. The aforementioned results were analyzed by statistical method, and contrasted between female and male. Results For the sensitivity, specificity, and negative prediction value of 64-DSCTCA in diagnosis of CAD, the differences were not statistically significant （P>0.05）, but positive prediction value was lower in female than that in male （P<0.05）. For the sensitivity, specificity, positive prediction value, and negative prediction value of 64-DSCTCA in diagnosis of coronary artery moderate stenosis, the differences were not statistically significant between female and male （P>0.05）. For the sensitivity, specificity, and negative prediction value of 64-DSCTCA in diagnosis of coronary artery severe stenosis and occlusion, the differences were not statistically significant （P>0.05）, but positive prediction value was lower in female than that in male （P<0.05）. Conclusions Diagnostic value of 64-DSCTCA is different in female and male. Positive prediction value of 64-DSCTCA is lower in female than that in male for diagnosis of CAD, coronary artery severe stenosis and occlusion.