|本期目录/Table of Contents|

洋地黄毒苷对裸鼠甲状腺乳头状癌组织生长的抑制作用

《现代肿瘤医学》[ISSN:1672-4992/CN:61-1415/R]

期数:
2020年21期
页码:
3670-3672
栏目:
论著（基础研究）
出版日期:
2020-09-29

文章信息/Info

Title:
The inhibitory effect of digitoxin on tissue growth of papillary thyroid carcinoma in nude mice
作者:
于明亮1; 段滨红2; 王 丹2; 杜馥曼2; 马金玲3; 赵凌斐2; 刘 余2
1.佳木斯大学，黑龙江 佳木斯 154007；2.黑龙江省医院内分泌科；3.神经呼吸重症医学科，黑龙江 哈尔滨 150036
Author(s):
YU Mingliang1; DUAN Binhong2; WANG Dan2; DU Fuman2; MA Jinling3; ZHAO Lingfei2; LIU Yu2
1.Jiamusi University，Heilongjiang Jiamusi 154007，China；2.Department of Endocrinology；3.Intensive Care Unit of Neurology and Respirology，Heilongjiang Provincial Hospital，Heilongjiang Harbin 150036，China.
关键词:
洋地黄毒苷; 甲状腺乳头状癌; 裸鼠
Keywords:
digitoxin; papillary thyroid carcinoma; nude mice
分类号:
R736.1
DOI:
10.3969/j.issn.1672-4992.2020.21.005
文献标识码:
A
摘要:
目的：探讨洋地黄毒苷对裸鼠甲状腺乳头状癌的治疗效果。方法：建立裸鼠肿瘤模型后，分为空白对照组、实验组，每组8只，分别给予生理盐水、洋地黄毒苷（1 mg/kg），隔日腹腔注射。治疗15天后，分析肿瘤体积，取下瘤体，选取实验组及空白对照组肿瘤组织进行HE染色。结果：治疗前，空白对照组与实验组裸鼠肿瘤体积分别为(109.43±7.67)mm3和(107.15±6.97)mm3。治疗15天后，空白对照组裸鼠肿瘤体积为（732.67±72.40）mm3，实验组裸鼠肿瘤体积为（251.91±27.29）mm3(实验组vs空白对照组，P<0.01）。HE染色结果示，实验组部分区域肿瘤细胞核淡染、缩小，且局部出现了变性、坏死。表明了洋地黄毒苷的抑瘤作用可能与其诱导肿瘤组织的破坏有关。结论：洋地黄毒苷可以抑制裸鼠体内甲状腺乳头状癌的生长。
Abstract:
Objective：To investigate the therapeutic effect of digitoxin on papillary thyroid carcinoma in nude mice.Methods：After nude mice models were established，they were divided into the blank control group(normal saline) and the experimental group(digitoxin，1 mg/kg)，with 8 nude mice in each group.Intraperitoneal injection was performed on every other day.After 15 days of treatment，tumor volume was analyzed.Then tumor was removed.Hematoxylin-eosin staining was performed in the experimental group and blank control group.Results：Before treatment，the tumor volume of the blank control group and the experimental group was(109.43±7.67)mm3 and (107.15±6.97)mm3，respectively.After 15 days of treatment，tumor volume in the blank control group was（732.67±72.40）mm3.Tumor volume in the experimental group was （251.91±27.29）mm3(experimental group vs blank control group，P<0.01）.Hematoxylin-eosin staining showed that tumor cells' nuclei in some areas of the experimental group was lightly stained and shrunk.Degeneration and necrosis occurred in some areas of the experimental group.It was suggested that the antitumor effect of digitoxin might be related to the destruction of tumor tissues.Conclusion：Digitoxin can inhibit the growth of papillary thyroid carcinoma in nude mice.

参考文献/References

［1］López-Lázaro M.Digitoxin as an anticancer agent with selectivity for cancer cells：Possible mechanisms involved［J］.Expert Opinion on Therapeutic Targets，2007，11(8)：1043-1053.
[2]Schneider NFZ，Cerella C，Simes CMO，et al.Anticancer and immunogenic properties of cardiac glycosides［J］.Molecules，2017，22(11)：1932.
[3]李盼丽，宋少莉.碘难治性分化型甲状腺癌靶向药物治疗进展［J］.国际放射医学核医学杂志，2016，40(3)：202-207. LI PL，SONG SL.Radioactive iodine refractory differentiated thyroid cancer targeted therapy［J］.International Journal of Radiation Medicine and Nuclear Medicine，2016，40(3)：202-207.
[4]石凌峰，胡渊，倪银星.NIS与甲状腺疾病的关系研究进展［J］.重庆医学，2018，47(06)：836-840. SHI LF，HU Y，NI YX.Advances in the relationship between NIS and thyroid disease［J］.Chongqing Medicine，2018，47(06)：836-840.
[5]李德宇，李娜.NIS、Oct-4和CD133在碘难治性分化型甲状腺癌中的表达分析［J］.医学理论与实践，2019，32(10)：1581-1583. LI DY，LI N.Expression analysis of NIS，oct-4 and CD133 in iodine-resistant differentiated thyroid cancer［J］.The Journal of Medical Theory and Practice，2019，32(10)：1581-1583.
[6]Cheng W，Liu R，Zhu G，et al．Robust thyroid gene expression and radioiodine uptake induced by simultaneous suppression of BRAFV600E and histone deacetylase in thyroid cancer cells［J］．Journal of Clinical Endocrinology ＆ Metabolism，2016，101(3)：962－971.
[7]De Morais RM，Sobrinho AB，De Souza Silva CM，et al.The role of the NIS(SLC5A5) gene in papillary thyroid cancer：A systematic review［J］.In J Endocrinol，2018，2018：1-11.
[8]Bastos AU，Oler G，Nozima BH，et al．BRAF V600E and decreased NIS and TPO expression are associated with aggressiveness of a subgroup of papillary thyroid microcarcinoma［J］．Eur J Endocrinol，2015，173(4)：525－540.
[9]Plantinga TS，Tesselaar MH，Morreau H，et al.Autophagy activity is associated with membranous sodium iodide symporter expression and clinical response to radioiodine therapy in non-medullary thyroid cancer［J］.Autophagy，2016，12(7)：1195-1205.
[10]Mukhopadhyay R，Venkatadri R，Katsnelson J，et al.Digitoxin suppresses human cytomegalovirus replication via Na+，K+/ATPase α1 subunit-dependent AMP-activated protein kinase and autophagy activation［J］.J Virol，2018，92(6)：e01861-e01867.
[11]Wang Y，Zhan Y，Xu R，et al.Src mediates extracellular signal-regulated kinase 1/2 activation and autophagic cell death induced by cardiac glycosides in human non-small cell lung cancer cell lines［J］.Molecular Carcinogenesis，2015，54(S1)：E26-E34.
[12]刘锋，陈志添，邵孔健，等.洋地黄毒苷对肺癌NCI-H446与A549细胞增殖和周期的影响［J］.癌变·畸变·突变，2015，27(01)：6-10. LIU F，CHEN ZT，SHAO KJ，et al.Effect of digitoxin on proliferation and cell cycle of NCI-H446 and A549 cells［J］.Carcinogenesis，Teratogenesis & Mutagenesis，2015，27(01)：6-10.
[13]Kaushik V，Yakisich JS，Azad N，et al.Anti-tumor effects of cardiac glycosides on human lung cancer cells and lung tumorspheres［J］.Journal of Cellular Physiology，2017，232(9)：2497-2507.
[14]Kaushik V，Azad N，Yakisich JS，et al.Antitumor effects of naturally occurring cardiac glycosides convallatoxin and peruvoside on human ER+ and triple-negative breast cancers［J］.Cell Death Discovery，2017，3：17009.
[15]Tesselaar MH，Crezee T，Swarts HG，et al.Digitalis-like compounds facilitate non-medullary thyroid cancer redifferentiation through intracellular Ca2+，FOS，and autophagy-dependent pathways［J］.Molecular Cancer Therapeutics，2017，16（1）：169-181.
[16]马金玲，王丹，段滨红，等.不同的洋地黄类药物对甲状腺乳头状癌细胞自噬的影响［J］.黑龙江医学，2019，43(5)：442-443. MA JL，WANG D，DUAN BH，et al.Digitalis-like compounds facilitating autophagy in papillary thyroid carcinoma［J］.Heilongjiang Medical Journal，2019，43(5)：442-443.
[17]Mijatovic T，Op De Beeck A，Van Quaquebeke E，et al.The cardenolide UNBS1450 is able to deactivate nuclear factor kappaB-mediated cytoprotective effects in human non-small cell lung cancer cells［J］.Molecular Cancer Therapeutics，2006，5(2)：391-399.

备注/Memo

备注/Memo:
黑龙江省自然科学基金面上项目（编号：H2015085）；黑龙江省卫生健康委员会科研课题（编号：2018091）
更新日期/Last Update: 1900-01-01