Objective: To observe the effect of VEGF-VEGFR pathway on EPCs function induced by Xuefu Zhuyu Tang. Method: After induced by serial concentrations of Xuefu Zhuyu Decoction-contained serum (XZDCS) and blank serum, EPCs proliferation, migration, adhension ability and VEGF, VEGFR expression were detected by MTT, Boyden chamber, adhension test and RT-PCR respectively. Result: Compared to the control group, 10% XZDCS could elevate the cell regeneration and adhension ability. The EPCs migration ability was inhibited by 5% XZDCS and improved by 10%,15% XZDCS. The higher two concentrations could up-regulate VEGF and VEGFR transcription significantly. Conclusion: Xuefu Zhuyu Tang could induce EPCs into angiogenesis by up regulating VEGF-VEGFR pathway to promote its proliferation, migration and adhension function.