Objective: To study pharmacodynamic effects of Qianlieshuang granule (QLSG) in treating chronic prostatitis from four aspects: anti-inflammation, analgesic, anticoagulation and promoting blood circulation to remove blood stasis, aiming at providing basis for clinical use. Method: Animals were randomly divided into model group, Qianlie Huichun group and QLSG groups (1.13,2.25,4.50 g·kg^-1). Xylene induced ear swelling method; acetic acid induced mouse peritoneal capillary permeability increased method; carrageenan induced rat paw swelling method and acetic acid writhing test in mice were used. Bleeding time (BT) in mice with cut tail, determination of the coagulation time (CT) by slide method, prothrombin time (PT) according to reagent box instruction (blood sampling via aorta in mice), BM-15A microscopic observation of mouse auricle capillary caliber and the capillary network intersection were applied to observe the effects of QLSG on anti-inflammation, analgesic, anticoagulation and promoting blood circulation to remove blood stasis. Result: QLSG could apparently restrain ear swelling induced by xylene (three doses of QLSG, P<0.01), decrease mouse peritoneal capillary permeability induced by acetic acid (middle and large doses of QLSG, P<0.01), restrain rat paw swelling induced by carrageenan (three doses of QLSG, P<0.01), decrease mice writhing induced by acetic acid (three doses of QLSG, P<0.01). With application of 2.25 or 4.50 g·kg^-1, QLSG could prolong PT, BT, and CT, and increase mouse auricle capillary caliber and the capillary network intersection (P<0.01). Conclusion: QLSG has the effect of anti-inflammation, analgesic, anticoagulation and function of promoting blood circulation to remove blood stasis.