Objective: To establish content determination method for gentiopicroside,naringin and protosappanin B from Xuanbi gel plaster. Method: HPLC was used with Merck RP-C₁₈ column (4.6 mm×250 mm, 5 μm), gradient eluted with mobile phase of 0.1% phosphoric acid-acetonitrile(0-33 min, 11% acetonitrile; 33-36 min,11% acetonitrile-20% acetonitrile; 36-67 min, 20% acetonitrile),column temperature was 20 ℃,flow rate was 0.6 mL·min^-1,detection wavelength 283 nm,injection volume was 10 μL. Result: The linear range of gentiopicroside, naringin and protosappanin B were 0.014 9-0.745 μg, 0.007 1-0.355 μg, 0.002 2-0.107 5 μg, respectively.Average recovery of these ingredients were 98.87% (RSD 2.19%), 103.51% (RSD 0.94%), 99.58% (RSD 2.49%),respectively. Conclusion: This established method was simple,accurate and repeatable,it could be used as quality control method for Xuanbi gel plaster.