Abstract:Objective To explore the effects of electroacupuncture assistant general anesthesia on myocardial reperfusion injury in rats.Methods Rat myocardial reperfusion mode was established, and the rats were randomly separated into control group and intervention group. The rats in two groups received general anesthesia. Besides, rats in intervention group were subjected to electroacupuncture at Baihui (DU20), Hegu (LI4), Neiguan (PC6), Zusanli (ST36) from 30 min before anesthesia induction to the end of operation. The blood of rats before surgery and 12h post-surgery was collected. The arachidonoylet-hanolamine (AEA), corticotrophin-releasing factor (CRF), CK-MB, cTnI, IL-1b, IL-6 and TNF-α in blood were measured by liquid chromatography-mass spectrometry technique or ELISA. The hearts of rats were harvest 12 h post-surgery, and the mRNA expressions of IL-1b, IL-6, TNF-α in hearts were measured by real-time PCR. Flow cytometry, TUNAL assay and western blot were used to evaluate the neutrophil counts, myocardial apoptosis and caspase 3 activation in heart tissue. Results The plasma AEA and CRF in rats of control group had no significant change before or after surgery, whereas the AEA and CRF in rats of intervention group increased significantly compared with before surgery and control group after surgery. Compared with before surgery, the CK-MB, cTnI, LDH, IL-1b, IL-6 and TNF-α increased significantly in two group, while the CK-MB, cTnI, LDH, IL-1b, IL-6 and TNF-α in intervention group post-surgery were lower than control group post-surgery. Flow cytometry and TUNEL assay showed that the infiltrated neutrophils, apoptotic cells and activated caspase 3 in rat heart tissue of intervention group decreased significantly compared with control group. Conclusion Electroacupuncture assistant general anesthesia inhibits the myocardial injury and inflammation response after myocardial ischemia reperfusion in rats.