Objective:To optimize β-cyclodextrin(β-CD) inclusion process of volatile oil from Xingqi Na'nihua granules. Method: β-CD inclusion complex was prepared by saturated aqueous solution method,inclusion process was optimized by uniform design with inclusion ratio of volatile oil and inclusion yield as indexes,taking ratio of volatile oil to β-CD,inclusion temperature and inclusion time as factors,and phase of this prepared inclusion complex was testified by XRD,DSC,IR,UV and TLC. Result: Optimum inclusion process was as follows:ratio of volatile oil to β-CD 1∶11,inclusion temperature at 60 ℃ and inclusion time 1 h.Inclusion ratio of volatile oil was as high as 96.84% under these optimized technology conditions.Validation result showed that inclusion compound had been formed. Conclusion: This optimized inclusion process was simple,stable and applicable to industrial production.