Objective: To investigate metabolites and metabolic dynamics of naringin by human intestinal microflora. Method: Human intestinal microflora were obtain from human feces,and incubated with naringin under anaerobic environment.HPLC was applied to monitor the concentration of naringin with mobile phase of methanol-0.1% phosphoric acid solution for gradient elution and detection wavelength at 250 nm.Metabolites were analysis by HPLC-MS. Result: Naringenin,one metabolite of naringin,was detected after incubation for 15 min,which was identified by HPLC-MS and its retention time was about 23 min in HPLC.Metabolism percentage of naringin was reached 100% after incubation for 120 min.Metabolic dynamics of naringin by human intestinal microflora was fitted with nonlinear kinetics.Naringenin could be converting to its metabolite through HPLC monitor. Conclusion: Naringin can be quickly metabolism by human intestinal microflora,its main metabolite is naringenin,secondary metabolites of naringin may be existed.